mouse anti human phosphor enos (Santa Cruz Biotechnology)
Structured Review

Mouse Anti Human Phosphor Enos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+human+phosphor+enos/pmc06868919-61-35-42?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 47 article reviews
Images
1) Product Images from "Crocetin promotes angiogenesis in human endothelial cells through PI3K-Akt-eNOS signaling pathway"
Article Title: Crocetin promotes angiogenesis in human endothelial cells through PI3K-Akt-eNOS signaling pathway
Journal: EXCLI Journal
doi: 10.17179/excli2019-1175
Figure Legend Snippet: The molecular formula of Crocetin (A). Relative viability measured by WST-1 in HUVECs exposed to various concentrations of Crocetin over a period of 72 h (B) (n=12). Measuring HUVECs viability in the combined regime of Crocetin, Wortmannin (a PI3K inhibitor) and NMA (an eNOS inhibitor) (C). Data are expressed as mean ± SD (n=12). *p<0.05, **p<0.01, ***p<0.001 using one-way ANOVA with Tukey post-hoc test.
Techniques Used:
Figure Legend Snippet: Gelatin zymogram showing MMP-9 and MMP-2 activities in HUVECs treated with Crocetin (A) (n=3). RT-PCR analysis of VEGF expression (B) (n=3). Western blot analysis of p-Akt/Akt ratio (C). Western blotting revealed the non-significant increase of p-Akt/Akt ratio during the 45 min-incubation of HUVECs with 50 µM Crocetin and these effects were diminished from time points 60 to 120 min. (n=3). Measuring the p-Akt/Akt and p-eNOS/eNOS ratios by ELISA (D, E, and F) (n=12) by using One-way ANOVA and Tukey's Post Hoc. * p <0.05; ** p <0.01; *** p <0.001.
Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Incubation, Enzyme-linked Immunosorbent Assay